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M94A0212.TXT
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1994-10-08
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Document 0212
DOCN M94A0212
TI Characterization of a kissing hairpin complex derived from the human
immunodeficiency virus genome.
DT 9412
AU Chang KY; Tinoco I Jr; Department of Chemistry, University of
California, Berkeley; 94720.
SO Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8705-9. Unique Identifier :
AIDSLINE MED/94360000
AB Base-pair formation between two hairpin loops--a kissing complex--is an
RNA-folding motif that links two elements of RNA secondary structure. It
is also a unique protein recognition site involved in regulation of
ColE1 plasmid DNA replication. The trans-activation response element
(TAR), a hairpin and bulge at the 5' end of the untranslated leader
region of the human immunodeficiency virus 1 mRNA, enhances the
transcription of the virus and is necessary for viral replication. Gel
electrophoresis and absorbance melting curves indicate that a
synthesized RNA hairpin (Tar*-16) with a loop sequence complementary to
the TAR loop sequence (CUGGGA) associates specifically with a
16-nucleotide TAR hairpin (Tar-16) to form a stable complex. RNase T1
probing indicates that the three guanines in the Tar-16 loop become
inaccessible in the complex. NMR imino proton spectra reveal that 5 base
pairs are formed between the two hairpin loops (Tar-16 and Tar*-16);
only the adenine at the 3' terminus of the TAR loop does not form a base
pair with the 5'-terminal uracil of the complementary loop. A
14-nucleotide hairpin [CCUA(UCCCAG)UAGG] with a loop sequence
complementary to the TAR loop is conserved within the gag gene of human
immunodeficiency virus 1. A synthesized RNA hairpin corresponding to
this conserved sequence also binds to the Tar-16 hairpin with high
affinity. It is possible that the same RNA loop-loop interaction occurs
during the viral life cycle.
DE Base Sequence Hydrogen Bonding HIV-1/*ULTRASTRUCTURE Molecular
Sequence Data Nuclear Magnetic Resonance Nucleic Acid Conformation
Regulatory Sequences, Nucleic Acid RNA, Viral/*ULTRASTRUCTURE Support,
U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).